Human IL-6 Matched Antibody Pair
The Human IL-6 Antibody Pair Kit includes pre-matched antibody pairs and standards, allowing for the development of customized enzyme-linked immunosorbent assays (ELISA) to detect and quantify human IL-6 protein levels. The kit comprises an unlabeled capture antibody, an HRP-conjugated detection antibody, and a standard protein. This Antibody Pair Kit, featuring mouse monoclonal antibodies, has the potential for quantifying both natural and recombinant human Interleukin-6 (IL-6) in ELISA and other immunoassays.
Components
* The kit provides raw materials for around 96 tests across 5 plates. However, actual results may vary based on the researcher's assay protocol and other variables.
Recommended Dilution and Reconstitution
• Human IL-6 Capture Antibody
Dilute 200X with PBS to the working concentration and add 100 μL/well.
• Human IL-6 Detection Antibody
Dilute 200X with PBS to the working concentration and add 100 μL/well.
• Human IL-6 Standard
Refer to the lot-specific CoA for reconstitution volume. Reconstitute each vial with 1% BSA in PBS. Standard curve using 2-fold serial dilutions is recommended.
Shipping Conditions:
Blue ice
|
Reagents
(Store at 2-8℃)
|
Quantity | Reconstitution |
| Human IL-6 Capture Antibody | 350 μL, 1 vial | Liquid |
| Human IL-6 Detection Antibody (HRP conjugated) |
350 μL, 1 vial | Liquid |
| Human IL-6 Standard | 5 ng/vial, 2 vials | Lyophilized |
Recommended Dilution and Reconstitution
• Human IL-6 Capture Antibody
Dilute 200X with PBS to the working concentration and add 100 μL/well.
• Human IL-6 Detection Antibody
Dilute 200X with PBS to the working concentration and add 100 μL/well.
• Human IL-6 Standard
Refer to the lot-specific CoA for reconstitution volume. Reconstitute each vial with 1% BSA in PBS. Standard curve using 2-fold serial dilutions is recommended.
Shipping Conditions:
Blue ice